Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 6;218(9):3098–3116. doi: 10.1083/jcb.201903124

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Jayadev et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — The PAT-2/PAT-3 integrin heterodimer is required for laminin-independent collagen recruitment to the pharyngeal BM. (A) Fluorescence images of the integrin β subunit PAT-3::mNG and the integrin α subunits PAT-2::2xmNG and INA-1::mNG in the pharynx. (B) Fluorescence images of pharyngeal BM collagen::mCh (left) and laminin::mNG (right) in control versus pat-3, pat-2, and ina-1 RNAi-treated 96-h adult animals. Quantification of collagen::mCh (control, n = 31; pat-3 RNAi, n = 36; pat-2 RNAi, n = 39; ina-1 RNAi, n = 37) and laminin::mNG (control, n = 21; pat-3 RNAi, n = 10; pat-2 RNAi, n = 24; ina-1 RNAi, n = 12) fluorescence intensity for all treatments is shown at the bottom. Bottom left: ***, P < 0.0001, one-way ANOVA followed by post hoc Dunnett’s test; n.s. (not significant), P > 0.05. Bottom right: n.s. (not significant), P > 0.05, one-way ANOVA. (C) A schematic outlining mosaic overexpression of PAT-2::mNG in the pharyngeal muscle cells and method for quantification of collagen levels is shown on top. A merged fluorescence image of myo-2p::PAT-2::mNG (green) and collagen::mCh (magenta) in a 72-h adult pharynx is shown on the bottom left. A spectral heatmap of collagen::mCh fluorescence intensity in the boxed region is shown on the bottom right. Quantification of the fold change in pharyngeal BM collagen::mCh fluorescence intensity upon PAT-2 overexpression is shown on the right (n = 25). ***, P < 0.0001, paired two-tailed Student’s t test. Box edges in boxplots depict the 25th and 75th percentiles, the line in the box indicates the median value, and whiskers mark the minimum and maximum values. Scale bars, 10 µm. A.U., arbitrary units.