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. 2019 Aug 8;218(9):2919–2944. doi: 10.1083/jcb.201902117

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© 2019 Bertero et al.

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Figure 8. — Correlation between altered chromatin compartmentalization and gene expression changes in lamin A/C haploinsufficient hiPSC-CMs. (A) Violin plots showing the expression of genes found with lamin A/C–sensitive domains. Boxplots indicate the first quartile, median, and third quartile, while whiskers are from the 5th to 95th percentiles. In the left panel, note that the tail of genes expressed at very low levels in corrected hiPSC-CMs is less pronounced in mutant cells. (B) Selected results from ontology enrichment analyses of up-regulated genes in domains aberrantly found in the A compartment in mutant hiPSC-CMs (average fold-change greater than two; Fig. S5 C). (C and D) RT-qPCR validation of gene expression changes in hiPSC-CMs matured by culture in vitro for 30 d (C) or by generation of 3D-EHTs (D). Differences versus mutant were calculated by one-way ANOVA with post hoc Holm–Sidak binary comparisons (*, P < 0.05; **, P < 0.01; n = 4 differentiations for panel C, and n = 3 3D-EHT batches for panel D; average ± SEM). RPKM, read kilobase per million mapped reads.