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. 2019 Aug 16;218(9):3077–3097. doi: 10.1083/jcb.201812073

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© 2019 Azoitei et al.

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Figure 1. — GEF-H1 contains an AID. (A) Domain organization and sequence alignment of GEF-H1 and Vav1, with AID of Vav1 shown in purple and the central Tyr residues in red. Secondary structure prediction (Ss pred) shown for GEF-H1 (gray). (B) RhoA exchange activity of recombinantly expressed GEF-H1 constructs with truncation or mutation of the AID. Red arrow indicates GEF addition. Curves show average of two independent experiments, and error bars display SD.