Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 24;218(9):2829–2840. doi: 10.1083/jcb.201904114

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Verma and Maresca

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — Branching MT nucleation is unaffected by D-TPX2 depletion. (A) Protein sequences of Xenopus (Xl) TPX2 and Drosophila (Dm) TPX2 (D-TPX2) were aligned using T-Coffee multiple alignment software (Notredame et al., 2000). Red dashed rectangle indicates a partially conserved CM1/γ-TuNA motif in D-TPX2. (B) Secondary structure prediction of D-TPX2 was generated using PSIPRED bioinformatics software (Buchan and Jones, 2019). Partially conserved CM1/γ-TuNA motif is expected to lie within α-helices (indicated with red dashed square). Conf, confidence; pred, predicted. (C) Western blot showing depletion of endogenous TPX2 with tubulin as a loading control (Ctrl). (D) A representative MT branching event in a D-TPX2–depleted cell coexpressing GFP-α-tubulin (green), mTurquoise2-Dgt5 (blue), and γ-tubulin-TagRFP (red). Time point 00:00 indicates landing of a Dgt5 molecule, which recruits γ-tubulin at 0:15 s. The Dgt5-γ-tubulin complex nucleates a MT branch at 0:35 s. Scale bar, 1 µm. (E) Distribution of branch angles in control and D-TPX2–depleted cells; n = 44. (F) Lifetime of branched daughter MTs in control and D-TPX2–depleted cells; n = 44. Time: min:s. Box plots indicate full range of variation (from minimum to maximum), the interquartile range, and the median. Two-tailed P values of Student’s t tests are reported; n.s., not significant or P > 0.05.