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. 2019 Aug 21;10:1923. doi: 10.3389/fmicb.2019.01923

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Copyright © 2019 Caimano, Groshong, Belperron, Mao, Hawley, Luthra, Graham, Earnhart, Marconi, Bockenstedt, Blevins and Radolf.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reciprocal regulation of OspC and OspA by RpoS occurs only within mammals and requires an intact RpoN/RpoS pathway. (A) Whole-cell lysates from wild-type strain B31 5A4 (WT; BbP1781), isogenic ΔrpoS mutant (BbP1752) and complemented mutant (comp; BbP1754) strains following temperature-shift in vitro and cultivation in DMCs separated by SDS-PAGE and stained with silver. (B) Whole cell lysates of wild-type strain 297 and isogenic ΔrpoS, ΔrpoN, and ΔbosR mutants following temperature-shift from 23 to 37°C in vitro and cultivation in DMCs. Molecular weight markers (kDa) are shown at the left of each gel.