TABLE 1.

The overview of setup for the Duplex assay.

	C. cayetanensis	Phocid herpesvirus 1 (PhHV-1)
Forward primer (5′ → 3′)	CyITS1_TT-F ATGTTTTAGCATGTGGTGTGGC	GGGCGAATCACAGATTGAATC
Reverse primer (5′ → 3′)	CyITS1_TT-R GCAGCAACAACAACTCCTCATC	GCGGTTCCAAACGTACCAA
Probe (5′ → 3′)	CyITS1_TT-P HEX-TACATACCCGTCCCAACCCTCGA-BHQ1	6FAM-TTTTTATGTGTCCGCCACCATCTGGATC-BHQ1
Primers conc.	0.5 μM	0.2 μM
Probe conc.	0.15 μM	0.1 μM
Amplicon size	141 bp	89 bp
Thermal profile	95°C for 3 min 1 × 95°C for 15 s 45 × 60°C for 30 s 45 ×