TABLE 3.
Values of the parameters obtained from the HPLC-analyzed peptidoglycans purified from the collections studied in this work.
| Collection | Relative abundance (mol%) of muropeptidea | Cross-linkb (%) | Peptidoglycan lengthc | ||||||
| Mono | Di | Tri | D-D | Lpp | Anh | Penta | |||
| PAO1 | 65.46 | 32.51 | 2.04 | 1.55 | 4.86 | 4.25 | 2.83 | 36.58 | 23.54 |
| PA14 | 66.08 | 32.37 | 1.56 | 1.73 | 3.75 | 4.25 | 1.50 | 35.48 | 24.11 |
| Bacteremia | 66.1 ± 2.6 (65.7)d | 31.9 ± 2.2 (32.1) | 2.0 ± 0.61 (2.2) | 1.0 ± 0.86 (1.0) | 5.6 ± 3.2 (5.1) | 5.3 ± 1.1 (4.9) | 1.8 ± 2.1 (0.9) | 35.9 ± 3.1 (36.5) | 20.3 ± 4.6 (20.9) |
| Early CF | 63.7 ± 4.1 (64.2) | 33.6 ± 3.0 (33.5) | 2.7 ± 1.3 (2.3) | 0.95 ± 1.3 (0.5) | 4.9 ± 1.4 (4.8) | 6.0 ± 1.6 (6.3) | 2.3 ± 1.7 (1.9) | 39.0 ± 5.3 (38.0) | 19.6 ± 6.5 (16.9) |
| Late CF | 64.9 ± 3.5 (65.8) | 32.8 ± 3.0 (31.9) | 2.2 ± 0.82 (2.0) | 1.0 ± 0.83 (0.8) | 5.0 ± 1.34 (4.4) | 5.6 ± 1.6 (5.5) | 3.0 ± 1.7 (2.4) | 37.2 ± 4.0 (36.2) | 19.8 ± 5.6 (18.3) |
| Collections’ mean ± SD | 64.9 ± 1.2 | 32.7 ± 0.85 | 2.3 ± 0.36 | 0.99 ± 0.02 | 5.1 ± 0.37 | 5.6 ± 0.35 | 2.3 ± 0.60 | 37.3 ± 1.55 | 19.9 ± 0.36 |
aMol%, molar fraction; Mono, monomers; Di, dimers; Tri, trimers; D-D, muropeptides having mDAP–mDAP crosslinking; Lpp, muropeptides bound to C-terminal Arg-Lys dipeptide of OprI lipoprotein; Anh, muropeptides having anhydro-1,6-anhydromuramic acid; Penta, muropeptides having a pentapeptide stem. bCross-link, degree of peptidoglycan cross-linking (percentage). cAverage number of disaccharide (N-acetylglucosamine and N-acetylmuramic acid) units in the sacculus sugar chains of each strain. dThe values in the table are the means ± SDs obtained from each parameter and collection, whereas the numbers within parenthesis correspond to the medians. The last row correspond to the mean values ± SDs calculated from all the parameters and collections.