FIGURE 3.

High-level ectopic expression of RelA^CTD impairs translation. Exponentially growing E. coli MG1655 cells harboring the CII-YFP plasmid were back-diluted to OD₆₀₀ 0.05, before the expression of relA, catalytically inactive G251E mutant relA^∗, or relA^CTD from high copy vector pMG25 was induced by addition of 1 mM IPTG (indicated by the arrow). (A) Growth, measured at 600 nm (OD₆₀₀) and (B) the fluorescence of YFP, measured at 520 nm was monitored every 15 min for 11 h. See section “Materials and Methods” for more details.