Table III.
Infecting species as detected by different methods. Only genera with relative abundance ⩾5% are listed
| Age (yrs)/sex/type/number | 16S metagenomic analysis | Targeted Sanger sequencing | Bacterial culture | |
|---|---|---|---|---|
| Genus | Species | |||
| 76/M/RK/Patient 1 | Staphylococcus | Staphylococcus aureus; Escherichia coli | Staphylococcus aureus | DTS: Staphylococcus aureus (3/3) |
| 71/F/RK/Patient 2 | Staphylococcus; Streptococcus; Escherichia | Staphylococcus aureus; Escherichia coli | Staphylococcus aureus; Sphingomonas aquatilis | DTS: Staphylococcus aureus (3/3) |
| 60/F/LK/Patient 3 | Streptococcus | Streptococcus dysgalactiae | Streptococcus dysgalactiae | DTS: Streptococcus dysgalactiae (1/4); BB-SY: Streptococcus dysgalactiae |
| 56/M/RK/Patient 4 | Staphylococcus; Streptococcus; Sphingomonas | Staphylococcus epidermidis; Bacteroides fragilis | Staphylococcus epidermidis/S. capitis; Streptococcus dysgalactiae | DTS: Staphylococcus caprae (3/3); BB-SY: Staphylococcus caprae |
| 74/M/LK/Patient 5 | Staphylococcus | Staphylococcus epidermidis | Staphylococcus epidermidis/S. caprae | DTS: Staphylococcus caprae (3/3); BB-SY: Staphylococcus caprae |
| 73/M/LK/Patient 6 | Staphylococcus; Pseudomonas; Klebsiella | Staphylococcus epidermidis | Staphylococcus epidermidis; Acinetobacter johnsonii | DTS: Staphylococcus epidermidis; BB-SY: Staphylococcus epidermidis |
| 62/M/RK/Patient 7 | Klebsiella; Pseudomonas | ND | Klebsiella pneumoniae | DTS: Klebsiella pneumoniae; BB-SY: Klebsiella pneumoniae |
| 62/M/LK/Patient 8 | Klebsiella; Pseudomonas; Escherichia | ND | ND | DTS: Klebsiella pneumoniae; BB-SY: NBG |
| 61/F/RH/Patient 9 | Staphylococcus | Staphylococcus epidermidis | ND | DTS: Staphylococcus caprae (3/3); SY: Staphylococcus caprae |
| 63/M/RH/Patient 10 | Serratia | Serratia marcescens | ND | DTS: NBG |
| 40/M/LH/Patient 11 | Sphingomonas; Propionibacterium | Streptococcus dysgalactiae; Staphylococcus epidermidis | ND | DTS: NBG (3/3) |
| Detection rate, n (%) | 11/11 (100) | 9/11 (82) | 7/11 (64) | DTS: 9/11 (82); BB-SY: 6/7 (85) |
RK, right knee; LK, left knee; DTS, deep tissue culture; BB-SY, synovial fluid culture on BD BACTEC Peds Plus (Becton, Dickinson and Company (BD), Sparks, Maryland); ND, not detected; NBG, no bacterial growth; RH, right hip; LH, left hip; SY, synovial fluid culture on BD BACTEC Plus Aerobic