Skip to main content
. Author manuscript; available in PMC: 2019 Sep 3.
Published in final edited form as: J Cell Physiol. 2019 Mar 25;234(10):18602–18614. doi: 10.1002/jcp.28498

FIGURE 2.

FIGURE 2

Effects of Hcy on the osteoblast mineralization and total ROS production. (a) Colonies formation unit of ALP (CFU-ALP) in MC3T3-E1 cells. (b) ALP activity analysis. (c) Alizarin Red-S staining shown calcified nodules formation. (d) Quantitative analyses of calcified nodules. (e) Confocal imaging of DCFDA fluorescence staining shown total ROS production in MC3T3-E1 cell lines. Values are means ± SD, n = 6, *p < 0.05, **p < 0.01 versus control. ALP: alkaline phosphatase; DCFDA: 2′,7′-dichlorodihydrofluorescein diacetate; Hcy: homocysteine; ROS: reactive oxygen species