Skip to main content
. Author manuscript; available in PMC: 2019 Sep 3.
Published in final edited form as: Nat Methods. 2019 Jan 28;16(2):183–190. doi: 10.1038/s41592-018-0305-7

Fig. 4 |. Target cell trogocytosis validates ligands for public F5- and 1G4-TCR.

Fig. 4 |

a, Schematic outline of trogocytosis used in the context of an A2-retricted library containing 12,055 epitopes. b, Histograms representing the mean fluorescence of second-round-sorted trogocytosis+ SCT-K562 cells after a validating co-incubation with F5-Jurkat or 1G4-Jurkat. SCT-K562 cells (eGFP+) were assessed for acquisition of TCR with an anti-mouse TCR (muTCR) antibody. Data are representative of two independent experiments. c, Representative FACS plots of 1G4-TCR or F5-TCR dextramer binding by sorted trogocytosis+ SCT-K562 cells (n = 1). d, Identification of enriched peptides after two rounds of trogocytosis selection by NGS. Rank average represents the ranking of enriched peptides, calculated based on the abundance of each peptide among all peptides, from experimental triplicates or quadruplicates