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. Author manuscript; available in PMC: 2019 Sep 3.
Published in final edited form as: Nat Methods. 2019 Jan 28;16(2):183–190. doi: 10.1038/s41592-018-0305-7

Fig. 5 |. Target cell trogocytosis identifies the cognate ligand for a neoantigen-specific, subject-derived TCR.

Fig. 5 |

a, Identification of enriched peptides after two rounds of trogocytosis selection by NGS. Rank average represents the ranking of enriched peptides, calculated based on the abundance of each peptide among all peptides, from experimental quadruplicates. b, Validation of TCR-neoantigen pairing via trogocytosis. Data are representative of two independent experiments. c, Cytotoxicity of neo-TCR T cells against mutUSP7-K562 or MART1-K562 cells in various effector-to-target (E/T) ratios (presented as percentage specific lysis). Data are presented as mean± s.e.m. and are representative of two independent experiments.