Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 27;10:1967. doi: 10.3389/fimmu.2019.01967

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Shathili, Bandala-Sanchez, John, Goddard-Borger, Thaysen-Andersen, Everest-Dass, Adams, Harrison and Packer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

CD52-Fc after fractionation by anion-exchange chromatography. (A) Anion exchange chromatography on a MonoQ-GL column fractionated the recombinant human CD52-Fc III into a gradient of anionic glycoforms displaying a spectrum of pI (see Supplementary Figure 2). (B) IFN–γ ELISpot assay with 2 × 10⁶ PBMCs in 200 μL/well incubated with no antigen or with anti-CD3/CD28 antibody Dynabeads in the presence of recombinant human CD52-Fc fractions (F29–F52; 5 μg/ml).