Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 1;33(17-18):1280–1292. doi: 10.1101/gad.325241.119

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Yeom and Groisman; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 5. — Acetylated HspQ inhibits ClpS-dependent proteolysis of ClpAP substrates in vivo. (A) Western blot analysis of crude extracts from oat-Flag phoP-HA (JY872), oat-FLAG phoP-HA hspQ (JY873), oat-Flag phoP-HA clpS (JY874), and oat-Flag phoP-HA hspQ clpS (JY875) Salmonella. (B,C) Western blot analysis of crude extracts (B) and mRNA abundance of the hspQ gene (C) from oat-Flag (JY655) Salmonella with no plasmid or with a hspQ-expressing plasmid (phspQ; pUHE-hspQ) using 0, 20, 50, 100, 200, 500, or 1000 µM IPTG. (D) Western blot analysis of crude extracts from oat-Flag (JY655), oat-Flag lon (JY1009), oat-Flag clpS (JY657), and oat-Flag lon clpS (JY1010) Salmonella. (E) Western blot analysis of crude extracts from oat-Flag (JY655), oat-Flag hspQ (JY686), oat-Flag lon (JY1014), and oat-Flag lon hspQ (JY1016) Salmonella. (F) Western blot analysis of crude extracts from oat-Flag (JY655) and oat-Flag hspQ (JY686) Salmonella with the plasmid vector (vector), the hspQ-expressing plasmid (phspQ), or the hspQ-Flag-expressing plasmid (phspQ-Flag) using 100 µM IPTG. (G) Western blot analysis of crude extracts from oat-Flag (JY655), oat-Flag clpS (JY657), oat-Flag hspQ (JY686), oat-Flag qad (JY774), oat-Flag clpS hspQ (JY687), oat-Flag hspQ qad (JY773), oat-Flag qad clpS (JY775), and oat-Flag clpS hspQ qad (JY772) Salmonella. (H) Western blot analysis of crude extracts from oat-Flag (JY655), oat-Flag clpS (JY657), oat-Flag hspQ (JY686), and oat-Flag clpS hspQ (JY687) Salmonella. (I) Western blot analysis of crude extracts from hspQ-Flag oat-Flag (JY865), hspQK96R-Flag oat-Flag (JY2002), and hspQK96Q-Flag oat-Flag (JY2003) Salmonella. Words with italicized letters indicate gene names. For Western blot analysis, samples were analyzed using antibodies directed to the Flag or HA epitopes or the AcnB, AtpB, OmpA, or GroEL proteins. Data are representative of at least two independent experiments, which gave similar results. See also Supplemental Figure S6.