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. 2019 Sep 1;33(17-18):1265–1279. doi: 10.1101/gad.327593.119

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© 2019 Hurtz et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 2. — Germline MLL is required for up-regulation of BCL6 expression during normal B-cell development. (A) Verification of deletion of Mll by genotyping. (B) Mll^fl/fl pre-B cells expressing EV or Cre were cultured in the presence of IL-7. qRT-PCR was performed to measure levels of Mll (day 7) and Bcl6 (days 7 and 21) following Cre-mediated deletion of Mll with or without IL-7 withdrawal. Withdrawal of IL-7 was carried out 24 h prior to RNA extraction. (C) Mll (day 7) and Bcl6 (days 7, 14, and 21) mRNA levels were measured in Mll^flfl spleen cells cultured in the presence of IL4, CD40L, and BAFF following Cre-mediated deletion of Mll. (D) qRT-PCR measurements of Mll (day 7) and Bcl6 (days 7 and 21) levels in Mll^fl/fl BCR-ABL1 B-ALL cells following Cre-mediated deletion of Mll. Cells were treated with vehicle control or 1 µmol/L imatinib 24 h prior to RNA extraction. All qRT-PCR measurements were performed using Hprt as a reference. Shown are mean values ± SD. n = 3.