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. 2013 Mar;182(3):1005–1020. doi: 10.1016/j.ajpath.2012.11.026

Figure 3.

Figure 3

A: DAPK and pSTAT3Y705expression pattern in DALM. Representative images of DAPK and pSTAT3Y705 staining in DALM tissue sections (n = 11) depicting heterogeneous pattern of expression are shown. Areas with high DAPK–high pSTAT3Y705 (filled arrowheads); high DAPK–low pSTAT3Y705 (open arrowheads); low DAPK–high pSTAT3Y705 (asterisks); and low DAPK–low pSTAT3Y705 (arrows) expression are indicated. Note the serial sections for DAPK and pSTAT3Y705 allowing direct comparison of expression in the same region of interest. TNF-induced functions in normal human colon epithelial cells: HCEC cells were stimulated with 0.66 ng/mL of TNF for various time points (1, 6, 24, 48, or 72 hours). DAPK (B) and STAT3 (C) expression/activation were assessed by immunoblotting using the corresponding antibodies. Representative Western blots of five independent experiments are shown. IL-6 (D) and IL-8 (E) secretion was measured in cell culture supernatants by ELISA. Data were obtained from more than five independent experiments performed in duplicate or triplicate. *P < 0.05 versus untreated control cells.