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. Author manuscript; available in PMC: 2019 Sep 3.
Published in final edited form as: Methods Mol Biol. 2019;1919:187–197. doi: 10.1007/978-1-4939-9007-8_14

Fig. 2.

Fig. 2.

Example of the results of one round of testing of combinations of various factors on differentiation of marmoset iPS cells. The concentrations shown in red in the table in the upper left were used in this round; other concentrations (initially level “3”) were used in prior rounds, or may be used in future rounds. Only two concentrations of dorsomorphin were used because the higher concentrations were toxic. Expression of the neuroectoderm marker genes SOX1 and NCAD, and the neural crest markers SOX10 and TFAP2A, was assessed. The y axis represents the mRNA level as Ct(gene)-Ct(β-actin); higher bars indicate higher levels of mRNA. In each case the first bar shows the value before the 6 days protocol and the other 2 or 3 bars show the values following the treatment with the factors as indicated in the table. Data are presented as means +/− SEM (n=3). * = p<0.05, ** = p<0.01, *** = p<0.001. See Note 9.