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. Author manuscript; available in PMC: 2019 Sep 3.
Published in final edited form as: Methods Mol Biol. 2019;1919:187–197. doi: 10.1007/978-1-4939-9007-8_14

Fig. 3.

Fig. 3.

Analysis of the effects of three small-molecule factors on genes that respond to activation of three different intracellular pathways in marmoset iPS cells. In each case “0” = mRNA level before treatment and “-” = level following 24 hours in basal medium only. DM = dorsomorphin at a range of concentrations from 0 to 2 μM; B = 40 ng/ml BMP4. BMP4 treatment induced a statistically significant upregulation of ID1 and ID3 mRNA; co-treatment with dorsomorphin resulted in lowered ID1, but not a statistically significant lowering of ID3. SB = SB4321542 at a range of concentrations from 0 to 3 μM; T = 10 ng/ml TGF-β1. Treatment with TGF-β1 increased expression of SERPINE and HES1, while SB431542 coadministration resulted in dosage-dependent reduction in expression of both genes. B = BIO at a range of concentrations from 0 to 2 μM. BIO increased the expression of Wnt/β-catenin targets SP5 and AXIN2. See Note 10.