Fig. 1.

Location of ECL Layer after density gradient centrifugation. ECL cells were isolated from the stomachs extracted from four rats with the esophageal and intestinal ends tied off. Each stomach was inverted and injected with pronase to form a stomach pouch. The ECL cells were collected and centrifuged following incubation with various solutions. A nycodenz gradient (conc. 6%) was applied to isolate an ECL layer of cells (black band), which yield approx 60% pure ECL cells. The collected ECL cells were incubated with Fluor-PACAP and sorted with FACS using parameters based first, on size and density, and second, on fluorescence. This process yielded approx 2 million pure ECL cells.