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. Author manuscript; available in PMC: 2019 Sep 3.
Published in final edited form as: J Mol Neurosci. 2005;26(1):85–97. doi: 10.1385/JMN:26:1:085

Fig. 9.

Fig. 9.

Calcium response to PACAP stimulation. Approximately 100,000 cells were attached to coverslips precoated with Cell-Tak, and Fluo-4 was added and incubated at 37°C for 30 min. The coverslips were mounted and observed under confocal microscopy and activated with 10−7 M PACAP at 160 s and 10−6 M ionomycin at 800 s. The panel on the left represents three individual experiments performed, whereas the right panel shows the average of 21 experiments of calcium response to PACAP-38 with standard deviation.