KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
phospho-p44/42 MAPK (Erk1/2) (20G11)	Cell Signaling Technology	Cat# 4376; RRID:AB_331772
phospho-p44/42 MAPK (Erk1/2) (E10)	Cell Signaling Technology	Cat# 9106; RRID:AB_331768
phospho-p44/42 MAPK (Erk1/2) (D13.14.4E)	Cell Signaling Technology	Cat# 4370; RRID:AB_2315112
P44–42 MAPK (Erk1/2)	Cell Signaling Technology	Cat#9102; RRID:AB_330744
CD11b (M1/70)	BioLegend	Cat# 101224; RRID:AB_755986
CD11b (M1/70.15)	Invitrogen	Cat# RM2817; RRID:AB_1464525
CD11c (N418)	BioLegend	Cat# 117324; RRID:AB_830649
PD-1 (J43)	eBioscience	Cat# 11–9985-85: RRID:AB_465473
PD-1 (29F.1A12)	Biolegend	Cat# 135215; RRID:AB_10696422
CTLA-4 (UC10–4B9)	ThermoFisher	Cat# 12–1522-81; RRID:AB_465878
CTLA-4 (UC10–4B9)	eBioscience	Cat# 17–1522-82; RRID:AB_2016700
LAG-3 (C9B7W)	BioLegend	Cat# 125209; RRID:AB_10639935
LAG-3 (eBioC9B7W)	eBioscience	Cat# 48–2231-82; RRID:AB_11149866
PD-L1 (10F.9G2)	BioLegend	Cat# 124311; RRID:AB_10612935
Armenian Hamster IgG (eBio299Arm)	ThermoFisher	Cat# 12–4888-81; RRID:AB_470073
CD3 (17A2, for flow cytometry)	BioLegend	Cat# 100214; RRID:AB_493645
CD3 (17A2, for flowcytometry)	BioLegends	Cat# 100221; RRID:AB_2057374
CD3 (145–2C11)	BD PharMingen	Cat# 561108/551163; RRID:AB_10562558/ RRID:AB_394082
EpCAM (G8.8)	BioLegend	Cat# 118215; RRID:AB_1236477
CD4 (GK1.5)	BioLegend	Cat# 100411; RRID:AB_312696
CD4 (GK1.5)	BioLegend	Cat# 100406; RRID:AB_312691
CD45 (30-F11)	BioLegend	Cat# 103108; RRID:AB_312973
CD8 (53–6.7)	BioLegends	Cat# 100734; RRID:AB_2075238
CD8 (5H19)	Invitrogen	Cat # MCD0817; RRID:AB_10374589
FoxP3 (FJK-16 s)	eBioscience	Cat# 17–5773-80; RRID:AB_469456
FoxP3 (FJK-16 s)	eBioscience	Cat# 25–5773-82; RROD:AB_891552
IFN-γ (XMG1.2)	BioLegend	Cat# 505825; RRID:AB_1595591
Ki-67 (16A8)	BioLegend	Cat# 652411; RRID:AB_2562663
Ki-67 (SolA15)	Invitrogen	Cat# 48–5698-82; RRID:AB_11149124
IgG1 (RTK2071)	BioLegend	Cat# 400415; RRID:AB_326521
IgG1 (RTK2071)	BioLegend	Cat# 400411; RRID:AB_326517
IgG2a (eBR2a)	eBioscience	Cat# 12–4321-81; RRID:AB_470051
IgG2a (eBR2a)	eBioscience	Cat# 17–4321-81; RRID:AB_470181
IgG2a (RTK2758)	BioLegend	Cat# 400521; RRID:AB_326542
IgG2a (RTK2758)	BioLegend	Cat# 400535; RRID:AB_10933427
TIM3 (RMT3–23)	eBioscience	Cat# 12–5870-81; RRID:AB_465973
Tbet (eBio4B10)	eBioscience	Cat# 12–5825-82; RRID:AB_925761
ICOS (c398.4A)	eBioscience	Cat# 11–9949-82; RRID:AB_465458
4–1BB (17B5)	eBioscience	Cat# 12–1371-83; RRID:AB_465865
GITR (DTA-1)	eBioscience	Cat# 48–5874-82; RRID:AB_1944394
OX40 (OX-86)	eBioscience	Cat# 17–1341-82; RRID:AB_10717260
CD62L (MEL-14)	eBioscience	Cat# 56–0621-82; RRID:AB_494003
CD44 (IM7)	BD PharMingen	Cat# 559250; RRID:AB_398661
Anti Fcgamma, purified	MSK Antibody and Bioresource Core	Clone: 2.4G2
CD3 (for in vitro T cell activation)	MSK Antibody and Bioresource Core	Clone: 145–2C11
CD28 (for in vitro T cell activation)	MSK Antibody and Bioresource Core	Clone: 37N
mouse anti-CTLA-4 (9H10, in vivo antibody)	Bioxcell	Cat# BE0131; RRID:AB_10950184
mouse anti-CTLA-4 (9D9, in vivo antibody)	Bioxcell	Cat# BE0164; RRID:AB_10949609
Syrian Hamster IgG	Bioxcell	Cat# BE0087; RRID:AB_1107782
InVivoMAb mouse IgG2b isotype control (MCP-11)	Bioxcell	Cat# BE0086; RRID:AB_1107791
mouse anti-PD-1 (RMP1–14, for in vivo experiment)	Bioxcell	Cat# BE0146: RRID:AB_10949053
InVivoMAb rat IgG2a isotype control (2A3)	Bioxcell	Cat# BE0089; RRID:AB_1107769
NK1.1 (PK136, in vivo antibody)	Bioxcell	Cat#BE0036; RRID: AB_1107737
Bacterial and Virus Strains
Ad5CMVCre	UI Viral Vector Core Web	VVC-U of Iowa-5
Chemicals, Peptides, and Recombinant Proteins
Selumetinib	Selleckchem	Cat# S1008
Trametinib	Selleckchem	Cat# S2673
gp100 peptide, human	Anaspec Inc	Cat# AS-62589
Recombinant mouse IL-2	eBioscience	Cat# 14–8021-64
Fixable viability dye (for flow cytometry)	eBioscience	Cat# 65–0866-14
Critical Commercial Assays
LIVE/DEAD Fixable Aqua Dead Cell Stain Kit	ThermoFisher	Cat# L34966
CD5 (Ly-1) MicroBeads, mouse	Miltenyi	Cat# 130–049-301
Luminex cytokine analysis (mouse)-MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay	Millipore	Cat# MCYTMAG-70K-PX32
Mouse Cytokine 23-plex Assay	Bio-Rad	#m60009rdpd
Cell titer Glo luminescent cell viability assay	Promega	G7571
QUICK RNA FFPE Kit	Zymo Research	R1008
Deposited Data
Affymetrix transcriptome data	NCBI Gene Expression Omnibus	GEO: GSE126202
Experimental Models: Cell Lines
Mouse; CL13	Dr. Phillip A. Dennis	PMID: 26637667
Mouse; CL25	Dr. Phillip A. Dennis	PMID: 26637667
Mouse; IO33	Dr. Phillip A. Dennis	PMID: 26637667
Mouse; HKP1	Dr. Vivek Mittal laboratory (WCMC)	PMID: 25704820
Mouse; LLC	ATCC	ATCC® CRL-1642
Mouse; KrasG12DTrp53fl/fl cell line from GEMM	Dr. Kwok-Kin Wong laboratory (NYU)	N/A
Mouse: primary T lymphocytes	This study	N/A
Experimental Models: Organisms/Strains
Mouse; KRASLSL-G12C GEMM	Dr. Kwok-Kin Wong laboratory (NYU)	PMID: 29945997
Mouse; Pmel TCR transgenic mouse	Dr. Nicholas Restifo laboratory (NIH)	PMID: 12925674
Mouse; Rag1–/–; B6.129S7-Rag1tm1Mom/J	Jax lab	Jax 002216
Mouse; C57BL/6J; C57BL/6J	Jax lab	Jax 000664
Software and Algorithms
3D slicer	Online download	https://www.slicer.org/
GraphGad Prism 7	Online download	https://www.graphpad.com/scientific-software/prism/
FlowJo v.10	Online download	https://www.flowjo.com/solutions/flowjo/downloads
IVIS Living image software v. 4.4	Perkin Elmer	http://www.perkinelmer.com/category/in-vivo-imaging-software?gclid=EAIaIQobChMIoK2N_LKW4AIVk4TICh0LeAU9EAAYASACEgLwr_D_BwE
Transcriptome Analysis Console (TAC) Software v.4.0	ThermoFisher Scientific	https://www.thermofisher.com/us/en/home/life-science/microarray-analysis/microarray-analysis-instruments-software-services/microarray-analysis-software/affymetrix-transcriptome-analysis-console-software.html
Robust Multi-array Average (RMA) procedure	Irizarry et al., 2003a	https://www.rdocumentation.org/packages/affy/versions/1.50.0/topics/rma
Other
MRI imaging	Dana-Farber Cancer Institute’s Lurie Family Imaging Center	http://www.lfic.dfci.harvard.edu/about
Affymetrix microarray Assay	ThermoFisher Scientific	Mouse Clariom D Pico assay