Figure 2:

Moving and merging operations of asters with dynamic light patterns. a, Asters are moved relative to the slide by repositioning the microscope stage. b, Overlay of five individual trajectories of aster movement relative to slide moving at 200 nm/s. The line represents the mean trajectory. Time lapse images show the position of the aster relative to the light pattern. l is the displacement of the aster from center of the light pattern. c, l versus stage speed. The dotted line at 400 nm/s represents the escape velocity. The red line is a linear fit. d, Illustration of the aster merge operation by a connected excitation pattern and the corresponding time series of images. e, Distance between merging asters over time for different initial separations. f, Maximum speeds of asters as measured from (e). The red line is a linear fit to the first three data points. In (c, e, f) the diamonds represent the mean of 5 experiments and the dots represent individual experiments.