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. 2019 Sep;60(12):3727–3739. doi: 10.1167/iovs.19-27453

Table.

Primer Sequence Used in Quantitative PCR


Gene

Species

Sequence (5′–3′)

Temperature (°C)
GAPDH Human ACA TCG CTC AGA CAC CAT G 55
TGT AGT TGA GGT CAA TGA AGG G
TNFα Human TGC ACT TTG GAG TGA TCG G 55
TCA GCT TGA GGG TTT GCT AC
IL-6 Human CCT TCC CTG CCC CAG TA 55
ATT CGT TCT GAA GAG GTG AGT G
CXCL-1 Human CTC TTC TTC CCT AGG AGC GT 55
TGT TCT TGG GGT GAA TTC CC
CCL2 (MCP-1) Human AGT GTC CCA AAG AAG CTG TG 55
AAT CCT GAA CCC ACT TCT GC