Table 1. Primers sequences, nested PCR and sequencing conditions.
Primer sequences | Activities | Procedure conditions | Size of PCR products |
---|---|---|---|
K1-F: 5’- cggagtgaccaaatctggga-3’ K4-R: 5’- gggaatctggtggtaacagc-3’ |
Nested PCR | 95°C×2 min, 30 cycles [95°C×30 sec, 60°C×90 sec, 72°C × 90 sec], 72°C ×10 min |
2097 bp |
K2-F: 5’- gccaagctgccattcatttg -3’ K3-R: 5’-gccttgttgaaagaagcaga-3’ |
95°C×2 min, 30 cycles [95°C×30 sec, 60°C×90 sec, 72°C × 90 sec], 72°C ×10 min |
850 bp | |
K2-F: 5’- gccaagctgccattcatttg -3’ K3-R: 5’-gccttgttgaaagaagcaga-3’ K5-F: 5’-ttatgtcattggtggaactaa-3’ K6-R: 5’-tctaggggtattcaaaggtgc-3’ |
Sequencing | ABI 3730XL automatic sequencer |