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. 2019 Sep 3;14(9):e0221895. doi: 10.1371/journal.pone.0221895

Table 1. Primers sequences, nested PCR and sequencing conditions.

Primer sequences Activities Procedure conditions Size of PCR products
K1-F: 5’- cggagtgaccaaatctggga-3’
K4-R: 5’- gggaatctggtggtaacagc-3’
Nested PCR 95°C×2 min, 30 cycles [95°C×30 sec,
60°C×90 sec, 72°C × 90 sec], 72°C ×10 min
2097 bp
K2-F: 5’- gccaagctgccattcatttg -3’
K3-R: 5’-gccttgttgaaagaagcaga-3’
95°C×2 min, 30 cycles [95°C×30 sec,
60°C×90 sec, 72°C × 90 sec], 72°C ×10 min
850 bp
K2-F: 5’- gccaagctgccattcatttg -3’
K3-R: 5’-gccttgttgaaagaagcaga-3’
K5-F: 5’-ttatgtcattggtggaactaa-3’
K6-R: 5’-tctaggggtattcaaaggtgc-3’
Sequencing ABI 3730XL automatic sequencer