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. 2019 Aug 21;17(8):e3000086. doi: 10.1371/journal.pbio.3000086

Fig 2. Aberrant synaptic growth and function along with disrupted social interactions in Sevo group mice.

Fig 2

(A) Representative images of basal dendritic spines in the somatosensory cortex of Control and Sevo groups at P21. Scale bar, 2 μm. (B) Quantification of the total (P < 0.001, unpaired t test) and mushroom (P = 0.039, unpaired t test) dendritic spine density in Control and Sevo groups at P21. (C) Three-dimensional reconstruction of mushroom spines (brown) and other morphologically immature spines (gray). Scale bar, 1 μm. (D) Quantification of total (P = 0.005, unpaired t test) and mushroom (P = 0.155, unpaired t test) synaptic density in mice of Control and Sevo groups. (E) Representative mEPSCs traces from L3-5 pyramidal neurons in the somatosensory cortex. (F) Cumulative distributions and average frequency (P = 0.007, Mann-Whitney test) of mEPSCs. (G) Cumulative distributions and average (P = 0.833, unpaired t test) of mEPSCs amplitude. (H) Representative eEPSCs traces from L3-5 pyramidal neurons from Control and Sevo mice at P21. The black dotted line (50 ms after the stimulation) indicated the measuring points for NMDAR-eEPSCs amplitudes. (I) Quantification of AMPAR/NMDAR ratio (P = 0.033, unpaired t test) from Control and Sevo groups. (J) Cartoon illustration for reciprocal social interaction test. (K) Quantification of the reciprocal social interaction test (following: P = 0.039, Mann-Whitney test; sniffing: P = 0.005; push-crawl: P = 0.578; unpaired t test) in pairing mice from Control and Sevo group. (L) Cartoon illustration for three-chamber test (social recognition). (M) Quantification of the social recognition test in mice of Control and Sevo groups (Control: P = 0.008; Sevo: P = 0.086; Mann-Whitney test). *P < 0.05; **P < 0.01; n.s, not significant. Data are shown as mean ± SEM. Underlying data are available in S1 Data. AMPAR, α-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor; eEPSC, evoked excitatory postsynaptic current; L3-5, layer 3–5; mEPSC, miniature excitatory postsynaptic current; NMDAR, N-methyl-D-aspartic acid receptor; n.s, not significant.