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. 2019 Aug 10;20(16):3901. doi: 10.3390/ijms20163901

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunohistochemical analysis of S-phase cells using anti-5′-bromo-2′-deoxyuridine (BrdU) antibody in the gastric mucosa of control (A) and VSL-treated (B) mice. Note that BrdU-labelled cells (brown nuclei) of VSL-treated tissues tend to appear more expanded when compared with control tissues and uniformly distributed in most of the glands. Magnification bar = 200 µm. Analysis of BrdU-labeled cell counts per gland in the mucosa of seven control and seven VSL-treated mice are presented as mean ± SE (C). No significant difference was found between VSL and control tissues.