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. 2019 Aug 9;20(16):3887. doi: 10.3390/ijms20163887

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Chemerin system expression in the porcine mediobasal hypothalamus (MBH) during the oestrous cycle. Gene and protein expression of chemokine-like receptor 1 (CMKLR1; A and B), G protein-coupled receptor 1 (GPR1; C and D) and C-C motif chemokine receptor-like 2 (CCRL2; E and F), as well as chemerin (RARRES2) mRNA content (G) in the porcine MBH on days: 2 to 3, 10 to 12, 14 to 16 and 17 to 19 of the oestrous cycle. Gene expression was determined by qPCR. Protein expression was determined by Western blotting; upper panels: representative immunoblots; lower panels: densitometric analysis of CMKLR1/GPR1/CCRL2 proteins relative to actin protein. Representative actin blots are identical for all chemerin receptors though proteins have been analysed on different gels. Results are presented as means ± SEM (n = 5). Bars with different superscripts differ (one-way ANOVA at p < 0.05 followed by Tuckey post-hoc test at p < 0.05).

Chemerin system expression in the porcine mediobasal hypothalamus (MBH) during the oestrous cycle. Gene and protein expression of chemokine-like receptor 1 (CMKLR1; A and B), G protein-coupled receptor 1 (GPR1; C and D) and C-C motif chemokine receptor-like 2 (CCRL2; E and F), as well as chemerin (RARRES2) mRNA content (G) in the porcine MBH on days: 2 to 3, 10 to 12, 14 to 16 and 17 to 19 of the oestrous cycle. Gene expression was determined by qPCR. Protein expression was determined by Western blotting; upper panels: representative immunoblots; lower panels: densitometric analysis of CMKLR1/GPR1/CCRL2 proteins relative to actin protein. Representative actin blots are identical for all chemerin receptors though proteins have been analysed on different gels. Results are presented as means ± SEM (n = 5). Bars with different superscripts differ (one-way ANOVA at p < 0.05 followed by Tuckey post-hoc test at p < 0.05).