Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 19;8(8):319. doi: 10.3390/antiox8080319

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Effect of GSH-LD on D_SH-Sy5Y cells treated with conditioned medium obtained from activated D_U937 cells. D_SH-Sy5Y cells pre-treated with GSH-LD (1 μM, 1 h) and exposed to conditioned medium obtained from activated D_U937 cells. After 24 h were conducted: (A) MTT assay; (B) Reactive oxygen species (ROS) production measured by DCF (2′,7′-dichlorofluorescin diacetate) fluorescence; (C) Measurement of ROS levels using NBT (Nitroblue tetrazolium) assay expressed as stimulation index; (D) Superoxide Dismutase (SOD) activity (E) Catalase (CAT) activity; (F) GSH level; (G) Glutathione Peroxidase (GPx) and H) Glutathione Reductase (GR) assay. Values are mean ± SD, n = 5; ^§ p < 0.05 vs. CTRL; * p < 0.05 vs. cells treated with conditioned medium.