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. 2019 Aug 19;20(16):4042. doi: 10.3390/ijms20164042

Figure 2.

Figure 2

ZnO NPs induce apoptosis in mouse Leydig TM3 cells. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay results of mouse Leydig TM3 cells treated with 0–8 μg/mL ZnO NPs for 24 h (A) or treated with 4 μg/mL ZnO NPs for 24~72 h (B). (C) The cells were treated with 0–4 μg/mL ZnO NPs for 24 h; then, the protein levels of cleaved Caspase-3, cleaved Caspase-8, Bcl 2, and Bax were investigated by Western blot; Actin was used as an internal control. (D) The relative protein levels were quantified by densitometry. (E) The cells were treated with 0 (a), 2 (b), 3 (c), 4 (d) μg/mL ZnO NPs for 24 h, then the AnnexinV-FITC positive staining cells were counted by flow cytometry. The experiment was done in triplicate and repeated three times. Data were analyzed by one-way ANOVA. * p < 0.05.