Figure 6.

Fear conditioning reveals considerable impairments in contextual and cue learning upon ether lipid deficiency: (A) The contextual fear conditioning paradigm was applied to WT and Gnpat KO mice, and the duration of the freezing response is shown as box plot, with whiskers indicating minimal and maximal values. Statistical analysis was performed using two-tailed Student’s t-test. (B) WT and Gnpat KO mice were exposed to the cued fear conditioning paradigm, and the duration of the freezing response was quantified before (“pre-CS”), during (“CS”), and after (“post-CS”) the conditioned stimulus (CS). Data are shown as mean ± SEM, and statistical analysis was performed using Mann–Whitney U tests with Bonferroni correction for the repeated measurements. The number of tested animals is given in brackets. The findings displayed in Figure 6A have been published previously with a smaller cohort of WT and Gnpat KO mice [11]. *** p < 0.001; n.s., not significant.