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. 2019 Aug 12;20(16):3924. doi: 10.3390/ijms20163924

Figure 5.

Figure 5

Effect of angiotensin II (Ang II) on miR-574-5p expression and EV secretion in human primary vascular smooth cells (VSMCs). (A) Relative expression of genes associated with synthetic or contractile phenotype measured by PCR in human VSMCs treated with anti-miR-574-5p or with Ang II (1 µM). (B) VSMCs treated or not with 1 µM of Ang II. (C) VSMCs treated with EV from normal VSMC or from VSMCs treated with 1 µM of Ang II. (D) Relative expression of miR-574-5p measured using PCR in VSMCs treated or not with Ang II. (E) Relative expression of miR-574-5p measured using PCR in EVs secreted by VSMCs treated or not with Ang II. Immunofluorescence and FISH staining of miR-574-5p (red); CD9, a membrane marker of EVs (green); fetuin, a marker of EV secreted from VSMC (yellow) and nucleus (blue) were used on primary human VSMCs. The yellow bar corresponds to 500 µm. Cells originate from an adult men. * p < 0.05; *** p < 0.001.