Table 2.

Primers used to detect virulence genes

Virulence Gene	Primer Sequence	Reference
hilA	F: 5′- CGTGAAGGGATTATCGCAGT −3′	Fardsanei et al., 2017 [25]
	R: 5′- GTCCGGGAATACATCTGAGC −3′
invA	F: 5′- ACAGTGCTCGTTTACGACCTGAAT − 3′	Fardsanei et al., 2017 [25]
	R: 5′- AGACGACTGGTACTGATCGATAAT − 3′
pefA	F: 5′- TTGCACTGGGTGGTTCTGG − 3′	Fardsanei et al., 2017 [25]
	R: 5′- TGTAACCCACTGCGAAAG − 3′
rck	F: 5′- AACGGACGGAACACAGAGTC − 3′	Fardsanei et al., 2017 [25]
	R: 5′- TGTCCTGACGAAAGTGCATC − 3′
sefA	F: 5′- GCAGCGGTTACTATTGCAGC − 3′	Fardsanei et al., 2017 [25]
	R: 5′- TGTGACAGGGACATTTAGCG − 3′
sipA	F: 5′- CCATTCGACTAACAGCAGCA − 3′	Fardsanei et al., 2017 [25]
	R: 5′- CGGTCGTACCGGCTTTATTA − 3′
sipC	F: 5′- AGACAGCTTCGCAATCCGTT − 3′	Fardsanei et al., 2017 [25]
	R: 5′- ATTCATCCCTTCGCGCATCA − 3′
sopB	F: 5′- CCTCAAGACTCAAGATG − 3′	Fardsanei et al., 2017 [25]
	R: 5′- TACGCAGGAGTAAATCGGTG − 3′
sopE	F: 5′- CGAGTAAAGACCCCGCATAC − 3′	Fardsanei et al., 2017 [25]
	R: 5′- GAGTCGGCATAGCACACTCA − 3′
spvC	F: 5′- ACTCCTTGCACAACCAAATGCGGA − 3′	Fardsanei et al., 2017 [25]
	R: 5′- TGTCTCTGCATTTCGCCACCATCA − 3′
ssaR	F: 5′- GTTCGGATTTGCTTCGG − 3′	Fardsanei et al., 2017 [25]
	R: 5′- TCTCCAGTGACTAACCCTAACCAA − 3′
ssrA	F: 5′- CTTACGATTACGCCATTTACGG − 3′	Fardsanei et al., 2017 [25]
	R: 5′- ATTTGGTGGAGCTGGCGGGACT − 3′
stnP1	F: 5′- TTGTCTCGCTATCACTGGCAACC − 3′	Fardsanei et al., 2017 [25]
	R: 5′- ATTCGTAACCCGCTCTCGTCC − 3′