Fig. 4.

Inhibition of CDV by T-705 at different time points after virus infection and reductions of viral titers by T-705. Cells were infected with both analyzed strains at a MOI of 0.1 and then incubated with different concentrations of T-705 at the indicated time points. Cells and the culture medium were collected at 96 h p.i. to determine viral titers (a-d) and calculate the inhibition percentages of T-705 (e-h). Untreated cells were served as controls. Percentages of inhibition were measured by comparing the viral titer of drug-treated to control cells. All experiments were carried out in triplicate. Statistical significance was analyzed by one-way ANOVA (Dunnett’s t-test). *, P < 0.01; **, P < 0.0001. ND means non-detectable