Fig. 5.

Comparisons of antiviral effects of T-705 and anti-CDV polyclonal serum. The Vero and DH82 cells were treated with T-705 (156.25 μg/ml), anti-CDV polyclonal serum (1:128) and a combination of T-705 (156.25 μg/ml) and anti-CDV polyclonal serum (1:128) at 0, 12, 24, 36 and 48 h p.i. with CDV-3 and CDV-11 at a MOI of 0.1. The mock groups were treated with DMEM p.i. at the same times. The virus titers in supernatant (b, d, f, h) were determined and compared with the viral titers in mock group. Viral replications in cells were detected by IFA and the mean fluorescence percentages (a, c, e, g) were calculated as the average of fluorescent areas under twenty randomly selected fields. All experiments were carried out in triplicate. Statistical significance was analyzed by one-way ANOVA (Dunnett’s t-test). *, P < 0.01; **, P < 0.0001