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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Biomaterials. 2019 Jun 5;216:119245. doi: 10.1016/j.biomaterials.2019.119245

Figure 3. Adult human salispheres in SM contain multiple cell types expressing progenitor and myoepithelial markers.

Figure 3.

(A) Confocal 10 μm section of stained D10 salispheres cultured in SM [FGF7] for epithelial marker β1 integrin (ITGB1), K5, αSMA, K14, KIT, and/or nuclei (DAPI, blue). Scale bar, 50 μm. (B) FACS analysis (% of cells) of KIT+, FGFR2b+, K5+ and K14+ cells present in D10 SM cultured salispheres. N=3 biological samples. Mean±SEM. (C) Gene expression of human D10 salispheres cultured in SM [FGF7] and BM, normalized to RPS29 and SM D3 (dotted line). *, P<0.05, one-way ANOVA with post-Dunnett test compared to D3, N = 4 biological samples.