Table 1.
Concentration [µM] that halved the antioxidant, anti-inflammatory and efflux pump modulating activity (IC50) of silychristin and its derivatives.
| Compound | ORAC IC50 [µM] |
CAA IC50 [µM] |
NO Production IC50 [µM] |
P-gp IC50 [µM] |
|---|---|---|---|---|
| Silychristin A | 5.4 ± 0.2 a | 11.4 ± 0.5 b,c | 65 ± 3 d | 21 ± 1 b |
| 2,3-Dehydrosilychristin A | 5.4 ± 0.1 a | 6.0 ± 0.2 a | 36.0 ± 0.2 b | 15.6 ± 0.5 a |
| Isosilychristin | 6.0 ± 0.1 a | 13 ± 2 c | 59.8 ± 0.8 c | 25.9 ± 0.4 c |
| Anhydrosilychristin | 5.77 ± 0.05 a | 8.0 ± 0.7 a, b | 22.5 ± 0.3 a | 16.8 ± 0.2 a |
ORAC—oxygen radical absorption capacity; CAA—cellular antioxidant activity assay; NO production—anti-inflammatory activity; P-gp—inhibition of P-glycoprotein (transmembrane efflux pump). Data are presented as the concentration (µM) that halved the respective activity (IC50); average of three (in the case of ORAC, only two) repetitions ± standard error of the mean. Letters indicate the differences between the groups (ANOVA followed by Duncan’s post hoc test, P < 0.05) within one assay; the different assays were evaluated independently on each other. Statistically significant levels were denoted by different letters.