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Normalized SERS spectra of (a) MPy-AgNPs and (b) BSA-modified MPy-AgNPs at pH 6–10. (c) Normalized SERS spectra zoomed into region of pH sensing bands. (d) pH calibration curves for MPy-AgNPs and BSA-modified MPy-AgNPs. Error bars are one standard deviation (N = 5 for BSA-modified MPy-AgNPs, N = 3 for MPy-AgNPs). Buffer for all samples was 1× PBS.
