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Measurements of BSA-modified MPy-AgNPs in 10 mM Tris-maleate with 0, 1, and 10 mM CaCl2. (a) SERS spectra without baseline correction or normalization. Inset focused on pH-sensitive bands. (b) pH calibration curves calculated from baseline-corrected Raman scattering spectra. (c) Intensity at 1095 cm−1 band used to gauge scattering amplification. (d) UV-vis data. CaCl2 concentrations in all plots indicated by color-legend in (b). Error bars are one standard deviation (N = 3).
