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. 2019 Aug 1;8(8):261. doi: 10.3390/antiox8080261

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Antioxidant activities of ethanolic extract (HE-ETH) on H₂O₂-treated HT22 neurons. HT22 was co-incubated with 250 μM H₂O₂ and 400 μg/mL HE-ETH before subjected to series of antioxidant assays. (A) Representative images of intracellular reactive oxygen species (ROS) stained by 2’,7’-dichlorodihydrofluorescin diacetate (DCFH-DA) with quantification of (B) ROS level, (C) catalase (CAT) activity and (D) glutathione (GSH) content. All values presented correspond to mean ± SD of triplicates (n = 3). Values of # p < 0.05; ## p < 0.01 compared with control, and * p < 0.05; ** p < 0.01 compared with H₂O₂-treated group were considered as statistically significant. Scale bar denotes 100 μm.

Antioxidant activities of ethanolic extract (HE-ETH) on H₂O₂-treated HT22 neurons. HT22 was co-incubated with 250 μM H₂O₂ and 400 μg/mL HE-ETH before subjected to series of antioxidant assays. (A) Representative images of intracellular reactive oxygen species (ROS) stained by 2’,7’-dichlorodihydrofluorescin diacetate (DCFH-DA) with quantification of (B) ROS level, (C) catalase (CAT) activity and (D) glutathione (GSH) content. All values presented correspond to mean ± SD of triplicates (n = 3). Values of # p < 0.05; ## p < 0.01 compared with control, and * p < 0.05; ** p < 0.01 compared with H₂O₂-treated group were considered as statistically significant. Scale bar denotes 100 μm.