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. 2019 Aug 1;8(8):261. doi: 10.3390/antiox8080261

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Improvement of mitochondrial functioning by ethanolic extract (HE-ETH) on H₂O₂-treated HT22 neurons. HT22 was co-incubated with 250 μM H₂O₂ and 400 μg/mL HE-ETH before subjected to series of assays to assess mitochondrial functions. (A) Representative images of mitochondrial membrane potential (MMP) stained by tetramethylrhodamine ethyl ester (TMRE) with quantification of (B) MMP, (C) mitochondrial toxicity and (D) ATP level. All values presented correspond to mean ± SD of triplicates (n = 3). Values of ## p < 0.01; #### p < 0.0001 compared with control, and * p < 0.05; *** p < 0.001; **** p < 0.0001 compared with H₂O₂-treated group were considered as statistically significant. Scale bar denotes 100 μm.

Improvement of mitochondrial functioning by ethanolic extract (HE-ETH) on H₂O₂-treated HT22 neurons. HT22 was co-incubated with 250 μM H₂O₂ and 400 μg/mL HE-ETH before subjected to series of assays to assess mitochondrial functions. (A) Representative images of mitochondrial membrane potential (MMP) stained by tetramethylrhodamine ethyl ester (TMRE) with quantification of (B) MMP, (C) mitochondrial toxicity and (D) ATP level. All values presented correspond to mean ± SD of triplicates (n = 3). Values of ## p < 0.01; #### p < 0.0001 compared with control, and * p < 0.05; *** p < 0.001; **** p < 0.0001 compared with H₂O₂-treated group were considered as statistically significant. Scale bar denotes 100 μm.