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. 2019 Aug 1;8(8):261. doi: 10.3390/antiox8080261

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of transcriptional expression measured by qPCR. HT22 was co-incubated with 250 μM H₂O₂ and 400 μg/mL ethanolic extract (HE-ETH) before subjected to qPCR for quantification of (A) nuclear factor erythroid 2-related factor 2 (Nrf2), (B) NAD(P)H quinone dehydrogenase 1 (NQO1) and (C) heme oxygenase-1 (HO-1) mRNA expression. All values presented correspond to mean ± SD of triplicates (n = 3).

Analysis of transcriptional expression measured by qPCR. HT22 was co-incubated with 250 μM H₂O₂ and 400 μg/mL ethanolic extract (HE-ETH) before subjected to qPCR for quantification of (A) nuclear factor erythroid 2-related factor 2 (Nrf2), (B) NAD(P)H quinone dehydrogenase 1 (NQO1) and (C) heme oxygenase-1 (HO-1) mRNA expression. All values presented correspond to mean ± SD of triplicates (n = 3).