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. 2019 Sep 2;20:689. doi: 10.1186/s12864-019-6056-8

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Overall workflow of the study: Along with positive and negative control samples, paired FFPE and fresh frozen samples went through different DNA extraction methods but same library preparation steps for Qiagen Breast Cancer Panel. After sequencing, bioinformatics analysis was conducted to examine coverages, variant calling and mutational signatures of called variants