Table 1.
Antibody/Probe | Cell expression | Fluorochrome | Emission spectra (nm) | Vendor |
---|---|---|---|---|
Anti-human CD3 | Pan expression T cell surface marker | APC | 650/660 | BD Biosciences (Franklin Lakes, NJ) |
Anti-human CD4 | Helper T cell surface marker | PE | 496/578 | BD Biosciences |
Anti-human CD8 | Cytotoxic T cell surface marker | FITC | 494/520 | BD Biosciences |
Anti-human CD19 | Pan expression B cell surface marker | PC7 | 496/785 | Beckman Coulter (Brea, CA) |
Live-dead discriminator | Compromised cell membrane marker | 7AAD | 546/647 | Thermo Fisher (Waltham, MA) |
CD cluster of differentiation, APC allophycocyanin, PE phycoerythrin, FITC fluorescein isothiocyanate, PC7 phycoerythrin cyanine 7, 7AAD 7-aminoactinomycin D