Table 2.
Antibody/Probe | Cell expression | Fluorochrome | Emission spectra (nm) | Vendor |
---|---|---|---|---|
Anti-human CD3 | Pan expression T cell surface marker | PC7 | 496/785 | BD Biosciences (Franklin Lakes, NJ) |
Anti-human CD56 | Natural killer cell surface marker | PE | 496/578 | BD Biosciences |
C. neoformans antibody 18B7 | IgG to C. neoformans capsule | AF488 | 490/525 | A kind gift of Dr. Arturo Casadevall |
Live-dead discriminator | Compromised cell membrane marker | PI | 493/636 | Molecular Probes |
CD cluster of differentiation, PC7 phycoerythrin cyanine 7PE, PE phycoerythrin, AF488 AlexaFluor 488, PI propidium iodide