Figure 3.

EBV Infection Induces B Cell Serine Transporter Expression, Uptake, and Catabolism

(A) LC-MS measurements of media serine concentrations of primary B cells cultured at the indicated DPI. Values indicate mean + SEM serine consumption over a 24-h period at the indicated time point, n = 3. ^∗p < 0.05 (paired two-tailed t test).

(B) Temporal plots of PM relative abundances of the neutral amino acid transporters ASCT1 and ASCT2.

(C) Flow cytometry of ASCT2 in uninfected B cells maintained in culture for 4 days or at 4 DPI with the indicated EBV strain at equal levels of infection. Representative of n = 3.

(D) Growth curves of newly infected primary B cells cultured in either replete media or media lacking serine, glycine or both serine and glycine. Data show the mean ± SEM, n = 3. ^∗p < 0.05; ^∗∗p < 0.01 (paired one-tailed t test).

(E) Schematic illustrating the fate of [2,3,3-²H]-serine in either the cytosolic (purple) or mitochondrial (red) one-carbon metabolic pathway. Enzymes involved in catabolizing serine are indicated in blue bold font.

(F) LC-MS measurements of dTTP isotopologues from 0 and 4 DPI cells grown in the presence of [2,3,3-²H]-serine. Data show the mean with SEM, n = 3.

See also Figures S5 and S6.