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. 2019 Apr 2;27(5):492–501. doi: 10.4062/biomolther.2018.199

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Copyright ©2019, The Korean Society of Applied Pharmacology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Hemolysis and lymphotoxicity assays. (A) Hemolytic activity of FX-compounds (5 μM) was determined by hemoglobin release from whole blood cells after 120 min incubation. Positive controls (=maximum lysis) were treated with 1% SDS. (B) Leukocytes were incubated in the presence of FX-compounds (5 μM) for a period of 48 and 72 h. Viability was analyzed using Calcein-AM and quantified in comparison to DMSO treated controls, which were set to be =100%. Results show data of six different healthy donors. Experiments were performed in triplicates. Values are given as the mean x-fold increase ± standard deviation.