Fig. 2.

RLYE blocks VEGF-A-induced angiogenic signaling in HRMECs. HRMECs were pretreated with or without RLYE (0.15 nM) for 30 min and stimulated with VEGF-A (10 ng/mL) for 15 min. (A) Cell lysates were separated by SDS-PAGE, followed by Western blotting to determine the protein and phosphorylation levels of Akt, ERK, FAK, and Src. (B–E) Levels of phosphorylated target genes were defined as a ratio of phosphorylated and non-phosphorylated proteins following protein quantitation using ImageJ software (NIH) (n=3). (F, G) Level of VEGFR2 phosphorylation was determined in cell lysates by Western blotting and quantified using ImageJ software (NIH) (n=3). ^**p<0.01.