Fig. 5.

RLYE binds to retinal microvascular endothelial cells in mice. (A) Mice were intravitreally injected with 2 μL of saline, VEGF-A (50 ng/2 μL), or RLYE (1.5 nM) 5 h prior to intravitreal administration of FITC-RLYE (2 μL of 1.5 nM). After 24 h, the mice were anesthetized and perfused with 4% paraformaldehyde. The retinas were isolated, flat-mounted, and stained with isolectin B4-647. The fluorescence of isolectin B4 and FITC-RLYE was determined by confocal microscopy. (B) The binding activity of RLYE to endothelium was quantified by measuring the fluorescence intensity of FITC-RLYE using ImageJ software (NIH) (n=5). Scale bar=100 μm. ^**p<0.01.