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. 2019 Aug 20;20(16):4062. doi: 10.3390/ijms20164062

Figure 4.

Figure 4

The effect of BA-12 on angiogenesis using a quail chick chorioallantoic membrane (qCAM) and human umbilical vein endothelial cells (HUVECs), and its biotoxicity based on a survival essay with Caenorhabditis elegans. (A) Schematic diagram of the experimental design. Con, control group treated with phosphate-buffered saline (PBS); Dis, dissolvent group treated with dissolvent containing 0.5% dimethyl sulfoxide (DMSO); Pos, positive control group treated with dovitinib (40 μg); BA-12 1–3, groups treated with BA-12 at doses of 20, 40, and 80 μg, respectively. (B) Inhibition of angiogenesis by BA-12 for qCAM assays in a dose-dependent manner. (C) Messenger RNA (mRNA) levels of vascular endothelial growth factor receptor 2 (VEGFR2) in qCAM samples. The most representative fields are shown. (D) Survival assay using Caenorhabditis elegans for biotoxicity detection. ANOVA with a post hoc test was used to calculate the significance of the differences; * p < 0.05, *** p < 0.001 compared with the dissolvent group. Experiments were executed three times. Results are displayed as means ± SD.