Figure 4.

Detection of non-transformants and HPT-overexpressing transgenic rice by RT-PCR and DAB staining. (A) Total RNA was isolated from leaves of 24 progenies of the T1 segregation population of OE-HPT-1. Rice Actin1 was an internal control. (B) H₂O₂ levels were detected by DAB staining in leaf segments of 24 T1 progeny of OE-HPT-1 treated with or without HyB for 12 h. (C) quantification of DAB staining results. The histogram tool in ImageJ was used to record the grayscale values of all pixels within the brown areas of eight-bit images. Each experiment was carried out in 4 technical replicates and 3 biological replicates. One representative experiment is shown. Data are mean ± SE. Bars with a same letter are not significantly different at P < 0.05.